

On November 13, 2018, Little was charged with the 1994 murder of Denise Christie Brothers in Odessa, Texas after having confessed the crime to a Texas Ranger in May 2018. On November 9, 2018, Little confessed to the 1996 fatal strangulation of Melissa Thomas. As of 2020, Little was serving a sentence at the California State Prison, Los Angeles County. On the day of the verdict, Little continued to insist on his innocence. On September 25, 2014, Little was found guilty and was sentenced to life imprisonment without possibility of parole. The prosecution presented the DNA evidence as well as testimony of witnesses who were attacked by the accused at different times throughout his criminal career. Little was tried for the murders of Elford, Nelson, and Apodaca in September 2014. In total, Little was tested for involvement in 93 murders of women committed in many US states. In connection with the new circumstances in Mississippi, the LaPree murder case was reopened.

A few months later, the police said that Little was being investigated for involvement in three dozen murders committed in the 1980s, which until then had been undisclosed. He was extradited to Los Angeles, where he was charged on January 7, 2013. All three women were killed and later found on the streets of Los Angeles. Little was arrested on September 5, 2012, at a homeless shelter in Louisville, Kentucky, and extradited to California to face a narcotics charge, after which authorities used Genealogical DNA testing to establish that he was involved in the murders of Carol Ilene Elford, killed on JGuadalupe Duarte Apodaca, killed on Septemand Audrey Nelson Everett, killed on August 14, 1989. Upon his release in February 1987, he immediately moved to Los Angeles and committed more than 10 additional murders. Little served two and a half years in prison for both crimes. One month later, he was found by police in the backseat of his car with an unconscious woman, also beaten and strangled, in the same location as the attempted murder of Barros. In October 1984, he was arrested for kidnapping, beating, and strangling 22-year-old Laurie Barros, who survived. Little moved to California, where he stayed in the vicinity of San Diego. Due to mistrust of witness testimonies, Little was acquitted in January 1984.

Prosecution witnesses identified Little in court as a person who spent time with Mount on the night before her disappearance.
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However, while under investigation, Little was transferred to Florida to be brought to trial for the murder of 26-year-old Patricia Ann Mount, whose body was found in September 1982.

A grand jury declined to indict him for her murder. In 1982, Little was arrested in Pascagoula, Mississippi, and charged with the murder of 22-year-old Melinda Rose LáPree, who had gone missing in September of that year. By 1975, he had been arrested 26 times in 11 states for crimes including theft, assault, attempted rape, fraud, and attacks on government officials. In 1961, Little was sentenced to three years in prison for breaking into a furniture store in Lorain he was released in 1964. Little claims that he took up boxing during his stints in prison, referring to himself as a former prizefighter. He has said he then "began traveling more widely and had more run-ins with the law”, being arrested in eight states for crimes that included driving under the influence, fraud, shoplifting, solicitation, armed robbery, aggravated assault, and rape. Little moved to Florida to live with his mother in his late 20s, working at various times as a cemetery worker and an ambulance attendant (by his own account). In 1956, after being convicted of breaking and entering into property in Omaha, Nebraska, Little was held in an institution for juvenile offenders. He attended Hawthorne Junior High School, where he had problems with discipline and achievement. Soon after his birth, Little's family moved to Lorain, Ohio, where he was brought up mainly by his grandmother. Samuel Little was born on June 7, 1940, in Reynolds, Georgia, to a mother he claimed was a prostitute.
